Part:BBa_K4849028

MtrCA - cytochrome protein complex

Metal-reducing microorganisms such as Shewanella oneidensis MR-1 and Geobacter metallireducens have evolved an innate EET pathway that makes them ideal for bioelectricity applications. The most characterised EET pathway in S. oneidensis MR-1 includes MtrCAB complex. Based on the current understanding, the electrons extracted from the oxidation of a substrate are ultimately transferred to the periplasmic decaheme cytochrome MtrA during anaerobic respiration. Subsequent electron transfer to the outer membrane β-barrel protein MtrB places MtrA in direct contact with MtrC. MtrC is re-oxidised by reducing terminal electron acceptors, such as electrodes, via direct or mediated electron transfer.

Image made in Biorender.com Image by: White, Gaye, et al. "Rapid Electron Exchange between Surface-Exposed Bacterial Cytochromes and Fe(III) Minerals." Proceedings of the National Academy of Sciences, vol. 110, no. 16, 2013, pp. 6346-6351

References:

1. Ross, Daniel E., et al. "Characterization of protein-protein interactions involved in iron reduction by Shewanella oneidensis MR-1." Applied and environmental microbiology 73.18 (2007): 5797-5808.

2. Breuer, Marian, et al. "Multi-haem cytochromes in Shewanella oneidensis MR-1: structures, functions and opportunities." Journal of The Royal Society Interface 12.102 (2015): 20141117.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 232